Bacteria in Water One Step Test Kit (for E. Coli) with free PH Litmus test – for PH Levels. 3 TESTS

Escherichia coli (E.coli) is the only member of the total coliform group of bacteria found only in the intestines of mammals, including humans. The presence of E.coli in water indicates recent faecal contamination and may indicate the possible presence of disease-causing pathogens, such as bacteria, viruses, and parasites. Although most strains of E.coli bacteria are harmless, certain strains may cause illness if a person is exposed to a contaminated water source. This can lead to gastrointestinal illness, skin, ear, respiratory, eye, neurologic, and wound infections. However the most commonly reported symptoms are stomach cramps, diarrhoea, nausea, vomiting, and low-grade fever. You should test your drinking water regularly, particularly in wells or other potentially exposed sources. Recommended guideline state that for every 100 ml of drinking water tested, no E.coli should be detected.

Specimen Collection & Test Preparation

Use only a clean and dry container (sterilise if possible) to collect the test sample. Prior to testing collect at least 1 tsp (4-5ml) of sample water and rest for 2 minutes. Only remove the test equipment from the packaging when ready to use and ensure the lysate solution is securely closed again after use.

Test Conditions

Ambient temperature between 20∘C−30∘C (optimum is 25∘C) – Relative humidity: $\le 80\%$

Test Procedure

1. Get all testing equipment ready (reagent strip, test tube, pipette and lysate solution).
2. Use the pipette to add 3 drops (0.4ml) of lysate solution to the test tube (measurements on side of tube).
3. Add 0.1ml of the sample water to the test tube (i.e. fill test tube to 0.5ml mark), SHAKE the mixture well.
4. Remove reagent test strip from the foil (do not touch test area), immerse strip into test tube for 10 mins.
5. Remove the test strip and compare results using the colour chart provided, note no colour change indicates that no E. Coli has been detected and the test is complete, otherwise see step 6 below.
6. Quantitate results:– If 10 min.